Indwara za allergie （Chemiluminescense Immunoassay）

Ibice bitandukanye bya allergens karemano bizatera umubiri gukora antibodi zitandukanye za IgE;nk'umukungugu wo mu nzu, hamenyekanye ibice birenga 30 bya allergique, birimo tropomyosine ya miti yo mu nzu, Enzymes zitandukanye, poroteyine zigenga, poroteyine zubaka, n'ibindi.;nk'amata, ibyingenzi byingenzi bya allergique ni casein, α-lactalbumin, β-lactoglobulin, nibindi.

Uburyo bwa gakondo bwo kwisuzumisha bukoresha ibiyikomokaho bya allerge, bipima "igiteranyo" cya IgE gihujwe na poroteyine zose muri allerge yose;kwisuzumisha ibice-byisesengura bifashisha allergen isanzwe cyangwa recombinant allergen ibice kugirango bamenye allergique yihariye IgE, bigatuma isuzuma ryindwara za allergique risobanuka neza.

Imikorere

Allergens yibigize isukurwa na chromatografi ya affinity na / cyangwa HPLC.Indangamuntu ya poroteyine hamwe na aside amine igenzurwa na mass spectrometrie hamwe nisesengura rya aside amine.Immune reactivité yemejwe na antibody ihuza monoclonal.Allergens isukuye nayo yemewe kuri IgE antibody ihuza.

Porogaramu:

Muri vitro ubushakashatsi bwibikorwa bya T-selile no kwerekana antigen

Ubwoko bwinyamanswa za IgE ibisubizo & asima

Ubudahangarwa bushya

Antibody detection, histamine irekura

Inyigisho zubatswe hamwe nubushakashatsi bwibinyabuzima